Antineoplastic Activity and Genotoxicity of Organic Extracts and Barbatic Acid Isolated from the Lichen Cladonia salzmannii Nyl

  • Joelma Pessoa Gonçalves
  • Mônica Cristina Barroso Martins
  • Maria de Lourdes Lacerda Buril
  • Jaciana Dos Santos Aguiar
  • Terezinha Gonçalves Da Silva
  • Talita Giselly dos Santos Souza
  • Noemia Pereira Da Silva Santos
  • Cristiano Aparecido Chagas
  • Eugênia Cristina Pereira
  • Emerson Peter da Silva Falcão Universidade Federal de Pernambuco
  • Nicácio Henrique Da Silva

Abstract

Background: Secondary metabolites are responsible for most of the biological activities of lichens. Many of these compounds exhibit significant antineoplastic activity. The aim of the present in vitro study was to evaluate the cytotoxic and genotoxic activities of organic extracts and purified barbatic acid from the lichen Cladonia salzmannii (Nyl.).


Methods: The thallus of the lichen (22 g) was cleaned and dried with the solvents diethyl ether, chloroform and acetone. Organic extracts were obtained using the hot exhausted method in a Soxhlet apparatus. Barbatic acid (BAR) was purified from the ether extract (1.3 g). Chemical analysis of the organic extracts and purified BAR was performed using thin-layer chromatography. The purity of purified BAR was determined using high-performance liquid chromatography. The MTT method [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and cytokinesis-block proliferation index (CBPI) were used to determine the cytotoxic activity of the organic extracts and purified BAR. The micronucleus test and comet assay were used to determine genotoxic potential of the organic extracts and purified BAR. Dimethyl sulfoxide was used as the diluting solvent of the samples in all biological tests.


Results: The IC50 results demonstrated significant cytotoxic potential of the ether extract (50 µg/mL) against cell lines NCI-H292 (IC50: 29.91 µg/mL), HEp-2 (IC50: 26.75 µg/mL) and HL-60 (IC50: 3.59 µg/mL) as well as the purified BAR (25 µg/mL) against cell lines HEp-2 (IC50: 15.79 µg/mL) and MCF-7 (IC50: 18.28 µg/mL). The CBPI demonstrated the cytotoxic activity of the purified BAR at all concentrations tested (5, 10, 20 and 40 µg/mL) and all organic extracts (50 µg/mL) against Ehrlich carcinoma cells. For sarcoma 180, only BAR purified at a concentration of 40 µg/mL and the ether and chloroform extracts (50 µg/mL) were considered cytotoxic. The micronucleus test revealed that the purified BAR at a concentration of 5 µg/mL had no genotoxic potential against either tumor cell line. Furthermore, the chloroform extract and purified BAR at a concentration of 10 µg/mL were not considered genotoxic for sarcoma 180. In the comet assay, all compounds tested induced DNA damage in both tumor lines.


Conclusion: Based on the present results, organic extracts and purified barbatic acid from C. salzmannii exhibit cytotoxic and genotoxic activity against of the tumor cell lines tested.

Author Biographies

Joelma Pessoa Gonçalves

Programa de Pós-graduação em Bioquímica e Fisiologia, UFPE, Recife-PE, Brasil

Mônica Cristina Barroso Martins

Departamento de Ciências Geográficas, UFPE, Recife-PE, Brasil

Jaciana Dos Santos Aguiar

Departamento de Antibióticos, UFPE, Recife-PE, Brazil.

Terezinha Gonçalves Da Silva

Departamento de Antibióticos, UFPE, Brazil.

Talita Giselly dos Santos Souza

Laboratório de Biodiversidade, Centro Acadêmico de Vitória, UFPE, Vitória de Santo Antão-PE, Brazil

Noemia Pereira Da Silva Santos

Laboratório NanoBioCel, Centro Acadêmico de Vitória, UFPE, Vitória de Santo Antão-PE, Brazil

Cristiano Aparecido Chagas

Laboratório de Biodiversidade, Centro Acadêmico de Vitória, UFPE, Vitória de Santo Antão-PE, Brazil

Eugênia Cristina Pereira

Departamento de Ciências Geográficas, UFPE, Recife-PE, Brazil

Nicácio Henrique Da Silva

Laboratório de Produtos Naturais, Departamento de Bioquímica, UFPE, Recife-PE, Brazil

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Published
2018-12-27
How to Cite
GONÇALVES, Joelma Pessoa et al. Antineoplastic Activity and Genotoxicity of Organic Extracts and Barbatic Acid Isolated from the Lichen Cladonia salzmannii Nyl. International Archives of Medicine, [S.l.], v. 11, dec. 2018. ISSN 1755-7682. Available at: <http://imedicalsociety.org/ojs/index.php/iam/article/view/2855>. Date accessed: 20 jan. 2019. doi: https://doi.org/10.3823/2594.
Section
Laboratory Medicine